Journal: bioRxiv

Article Title: RocS drives chromosome segregation and nucleoid occlusion in Streptococcus pneumoniae

doi: 10.1101/359943

Figure Lengend Snippet: Detection of CPS and DNA in (a) cpsD-3YE and cpsD-3YE-ΔrocS and (b) cpsD-3YF and cpsD-3YF-ΔrocS . Phase contrast (grey), CPS (red), DAPI (blue) and overlays are shown on the left. White arrows show CPS production defects, white arrowheads show anucleate cells and black arrowheads show nucleoid segregation defects. Scale bar, 1 µm. The corresponding percentage of anucleate cells are shown in the middle. (Two population proportions test: **** P < 0.0001). The corresponding distribution of the cell length are shown on the right. nT indicates the number of cells analyzed from 3 independent experiments and standard errors are indicated with error bars.

Article Snippet: Construction of the pGBDU- cpsD bait plasmid and expressing CpsD fused to the DNA-binding domain of Gal4 (BD) was described in ( ).

Techniques:

Journal: bioRxiv

Article Title: RocS drives chromosome segregation and nucleoid occlusion in Streptococcus pneumoniae

doi: 10.1101/359943

Figure Lengend Snippet: When CpsD is autophosphorylated, capsule is properly produced and RocS and ParB actively contribute to chromosome segregation (viable encapsulated progeny). By contrast, non-phosphorylated CpsD hinders both capsule synthesis and chromosome segregation inducing a division block. The deletion of rocS alleviates the division block and results in uncontrolled cell constriction with improper chromosome segregation (pinching and asymmetric distribution) leading to non-viable progeny. ParB, RocS, CpsD and its transmembrane activator CpsC are indicated by blue, yellow, brown and pink circles, respectively. Red "P" and the turquoise star indicate CpsD autophosphorylation and the oriC region, respectively. Capsule is shown in light (new capsule produced during cell division) and dark (inherit from the mother cell) red.

Article Snippet: Construction of the pGBDU- cpsD bait plasmid and expressing CpsD fused to the DNA-binding domain of Gal4 (BD) was described in ( ).

Techniques: Produced, Blocking Assay